Table 3 Comparison of anti-CD5 immunohistochemistry staining intensity on tissues fixed with and without ultrasound

From: Ultrasound-accelerated formalin fixation of tissue improves morphology, antigen and mRNA preservation

Microwave antigen retrieval

IHC stain reactions a

Formalin fixation

Formalin fixation with ultrasound

  

30 min

6 h

22 h

15 min

30 min

Yes

Long

++

+++

++

++++

+++

No

Long

+

+++

+

Yes

Short

+

++

+++

+++

No

Short

+

  1. aLong IHC staining means 18-h primary antibody, 30-min secondary antibody, and 30-min ABC. Short IHC staining means 10-min primary antibody, 5-min secondary antibody, and 5-min ABC.
  2. 4+, strongest; 3+, strong; 2+, moderate; 1+, weak; −, no staining.