Figure 3

Dual-color FISH of ERBB2 (orange signals) and Myc (green signals) (a–e), and EGFR (orange signals) and Myc (green signals) (f and g). Coamplified signals were found in single nuclei in various combinations of amplified patterns: Myc was amplified in LC and ERBB2 in MS in case 3 (a), and Myc in MS and EGFR in LC in case 13 (b). Case 13 also had Myc-amplified cells without ERBB2 amplification (c). In cases 10 and 16, through the triple-band filter Myc signals (green fluorescence) were not well discernible (d and f), but using a SpecrumGreen™-specific filter, the green fluorescence of the Myc gene was clearly observed (e and g). The number and distribution of Myc and ERBB2 were almost the same in case 10 (d and e). In case 16, EGFR (orange signals) and Myc (green signals) were amplified in MS, however, differently distributed in the nuclei (f and g).