FIGURE 2

Representative photomicrographs illustrating immunohistochemical abnormalities in various histologic categories of PanIN lesions. The protein of interest is designated in rows, and the PanIN histologic category is designated in columns. Row 1, absence of nuclear p53 labeling in PanIN 1A, 1B, and 2, with diffuse nuclear labeling in PanIN 3. Row 2, absence of nuclear MIB-1 labeling in PanIN 1A and 1B, with focal nuclear labeling in PanIN 2 and 3. Row 3, absence of MUC1 labeling in PanIN 1A and 1B; note the apical labeling in normal ducts subjacent to PanIN lesions. In contrast, strong and diffuse apical labeling is seen in PanIN 2 and 3 lesions. Row 4, unlike the case with MUC1, MUC5 labeling is present in low-grade PanIN 1A and 1B lesions, with persistent expression in PanIN 2 and 3 lesions. Row 5, absence of fascin expression in PanIn1A, with focal or diffuse labeling in subsequent PanIN 1B, 2 and 3 lesions; note intense diffuse cytoplasmic labeling in PanIN 3 lesion. Row 6, absence of 14-3-3σ labeling in PanIN 1A and 1B lesions, with expression in PanIN 2 and 3 lesions.