Abstract
The pacemaker cells of the heart initiate the heartbeat, sustain the circulation, and dictate the rate and rhythm of cardiac contraction1. Circulatory collapse ensues when these specialized cells are damaged by disease, a situation that currently necessitates the implantation of an electronic pacemaker2. Here we report the use of viral gene transfer to convert quiescent heart-muscle cells into pacemaker cells, and the successful generation of spontaneous, rhythmic electrical activity in the ventricle in vivo. Our results indicate that genetically engineered pacemakers could be developed as a possible alternative to implantable electronic devices.
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References
Brooks, C. M. & Lu, H.-H The Sinoatrial Pacemaker of the Heart (Thomas, Springfield, Illinois, 1972).
Kusumoto, F. M. & Goldschlager, N. N. Engl. J. Med. 334, 89–97 (1996).
Wobus, A. M., Rohwedel, J., Maltsev, V. & Hescheler, J. Ann. NY Acad. Sci. 752, 460–469 (1995).
Kubo, Y., Baldwin, T. J., Jan, Y. N. & Jan, L. Y. Nature 362, 127–333 (1993).
Herskowitz, I. Nature 329, 219–222 (1987).
Slesinger, P. A. et al. Neuron 16, 321–331 (1996).
Irisawa, H., Brown, H. F. & Giles, W. Physiol. Rev. 73, 197–227 (1993).
Santoro, B. & Tibbs, G. R. Ann. NY Acad. Sci. 868, 741–764 (1999).
Imoto, Y., Ehara, T. & Matsuura, H. Am. J. Physiol. 252, 325–333 (1987).
Hirano, Y. & Hiraoka, M. J. Physiol. (Lond.) 395, 455–472 (1988).
Rodriguez-Contreras, A., Nonner, W. & Yamoah, E. N. J. Physiol. (Lond.) 538, 729–745 (2002).
Campbell, D. L., Giles, W. R. & Shibata, E. F. J. Physiol. (Lond.) 403, 239–266 (1988).
Brown, H. F., Kimura, J., Noble, D., Noble, S. J. & Taupignon, A. Proc. R. Soc. Lond. B 222, 329–347 (1984).
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Miake, J., Marbán, E. & Nuss, H. Biological pacemaker created by gene transfer. Nature 419, 132–133 (2002). https://doi.org/10.1038/419132b
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DOI: https://doi.org/10.1038/419132b
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