Early work on the function of CD95, an interview with Shige Nagata

Nagata, S

doi:10.1038/sj.cdd.4401453

Interview
Published: 14 May 2004

Early work on the function of CD95, an interview with Shige Nagata

S Nagata^1,2

Cell Death & Differentiation volume 11, pages S23–S27 (2004)Cite this article

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This interview is part of a series of articles to mark the 10th anniversary of Cell Death and Differentiation.

As a Ph.D. student (1972–1977) at the University of Tokyo, I worked on an enzyme called ‘Polypeptide chain elongation factor 1 (EF-1)’. I purified this enzyme from pig liver and characterized it biochemically.^{1, 2} During this period, recombinant DNA technology was developed in the USA and Europe.^{3, 4} I thought that this new technique was revolutionary and would change the way Bioscience was conducted. Since nobody in Japan was working with the recombinant DNA technology at that time, I decided to go to Dr. Charles Weissmann’s laboratory in Switzerland in 1977. I first learned recombinant DNA technology using Qβ phage, and then I became engaged in cloning of the cDNA for human leukocyte interferon (IFNα). After a year of hard work, we found an Escherichia coli clone producing human IFNα on December 24, 1979.^{5, 6}

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Acknowledgements

Professor Shigekazu Nagata is grateful to the funding bodies that supported this work, in particular, the City of Osaka, Ministry of Education, Science, Sports and Culture in Japan, and Japan Science Technology Co. He is indebted to many colleagues who contributed help, reagents, ideas and discussions, but Shige Nagata would especially like to acknowledge the help, insight, and friendship of Dr. Pierre Golstein.

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Department of Integrated Biology, Graduate School of Frontier Bioscience, Osaka University, 2-2 Yamada-oka, Suita, Osaka, 565-0871, Japan
S Nagata
Department of Genetics, Graduate School of Medicine, Osaka University, 2-2 Yamada-oka, Suita, Osaka, 565-0871, Japan
S Nagata

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Nagata, S. Early work on the function of CD95, an interview with Shige Nagata. Cell Death Differ 11 (Suppl 1), S23–S27 (2004). https://doi.org/10.1038/sj.cdd.4401453

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Published: 14 May 2004
Issue date: 01 July 2004
DOI: https://doi.org/10.1038/sj.cdd.4401453

Early work on the function of CD95, an interview with Shige Nagata

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