Dear Editor,

To test this hypothesis, we examined the ability of a number of agents to induce apoptosis in parental and caspase-6−/− DT40 chicken lymphoma cells.3 Our data revealed that the ability of the topoisomerase II poison etoposide to induce apoptosis in the caspase-6−/− cell line was intact (Figure 1b) despite the absence of detectable procaspase-6 protein (Figure 1c), ruling out the possibility that the gene targeting and selection process had raised the apoptotic threshold in this cell line. When treated with camptothecin, the parental and caspase-6−/− cells displayed roughly equal sensitivity to the induction of apoptosis as assessed by the appearance of cells with subdiploid DNA content after propidium iodide staining (Figure 1d) or the activation of caspase 3-like activity (Figure 1e).

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