Figure 2
Expression of prostrate stromal 20 (ps20) induces opposing effects on stromal- and tumour-derived prostate cancer (PCa) cells. (A–D) PC-3 (A), DU145 (B) or WPMY-1 (C) and LNCaP (D) cells transduced to express ps20FL or ps20TR were assessed for proliferation over time using MTS viability assay. (E and F) Transduced WPMY-1 (E) or LNCaP (F) cells were treated with RNAse and cell cycle staging was elucidated by propidium iodide (PI) staining followed by Watson analysis on FlowJo. (G and H) Transduced WPMY-1 (G) or LNCaP (H) cells were stained with PI and annexin V and cells were analysed using FACS to enumerate the percentage of cells undergoing apoptosis. Means and s.e.m.s of three experiments. *P<0.05, **P<0.01 and ***P<0.001 by Student’s T-test.
