Figure 3 | British Journal of Cancer

Figure 3

From: Expression of two WFDC1/ps20 isoforms in prostate stromal cells induces paracrine apoptosis through regulation of PTGS2/COX-2

Figure 3

Conditioned media from WPMY-1 cells expressing prostrate stromal 20 (ps20) inhibits growth and induces apoptosis in of prostate cancer (PCa) cells. (A–D) Conditioned media taken from transduced WPMY-1 following 72 h in culture was titrated onto PC-3 (A), DU145 (B), LNCaP (C) and WPMY-1 (D) cells and cultured for 96 h. Viability was measured by MTS viability assay and plotted as a percentage of cells grown in complete media only. (E–H) DU145 (E and F) or PC3 (G and H) cells were cultured in WPMY-1 CM (three different batches) for 48 h and then stained with propidium iodide (PI) and annexin V to enumerate the percentage of cells undergoing apoptosis. Representative plots (E–G) and means and s.e.m. of three experiments (F and G) are shown. *P<0.05 by Student’s T-test.

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