Abstract
Different cardiac stem/progenitor cells have been recently identified in the post-natal heart. We describe here the identification, clonal expansion and characterization of self-renewing progenitors that differ from those previously described for high spontaneous cardiac differentiation. Unique coexpression of endothelial and pericyte markers identify these cells as cardiac mesoangioblasts and allow prospective isolation and clonal expansion from the juvenile mouse ventricle. Cardiac mesoangioblasts express many cardiac transcription factors and spontaneously differentiate into beating cardiomyocytes that assemble mature sarcomeres and express typical cardiac ion channels. Cells similarly isolated from the atrium do not spontaneously differentiate. When injected into the ventricle after coronary artery ligation, cardiac mesoangioblasts efficiently generate new myocardium in the peripheral area of the necrotic zone, as they do when grafted in the embryonic chick heart. These data identify cardiac mesoangioblasts as committed progenitors, downstream of earlier stem/progenitor cells and suitable for the cell therapy of a subset of juvenile cardiac diseases.
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Abbreviations
- ANF:
-
atrial-natriuretic-peptide
- AP:
-
alkaline phosphatase
- SMA:
-
smooth α actin
- vWF:
-
von Willebrand factor
- CAL:
-
coronary artery ligation
- HH:
-
Hamilton Hamburger
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Acknowledgements
This work was supported by grants from Fondation Leducq, European Community (European Vascular Genomics Network, Heart Repair, Nax and Normacor), Italian Ministries of Health and Research, Firb and CARIPLO. Beatriz G Galvez was supported by a Program 3+3 Fellowship from the Centro Nacional de Investigaciones Cardiovasculares (CNIC), Spain. We thank the microscope service of the san Raffaele foundation for help with the images.
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Galvez, B., Sampaolesi, M., Barbuti, A. et al. Cardiac mesoangioblasts are committed, self-renewable progenitors, associated with small vessels of juvenile mouse ventricle. Cell Death Differ 15, 1417–1428 (2008). https://doi.org/10.1038/cdd.2008.75
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DOI: https://doi.org/10.1038/cdd.2008.75
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