Figure 3 | Cell Death & Disease

Figure 3

From: Enhancement of auranofin-induced lung cancer cell apoptosis by selenocystine, a natural inhibitor of TrxR1 in vitro and in vivo

Figure 3

Contribution of ERK and AKT pathways to combined treatment-induced apoptosis. (a) SeC enhances AF-induced inactivation of ERK and AKT. The protein expression were detected by western blotting method. (b) ERK and AKT inhibitors enhance combined treatment-induced growth inhibition against A549 cells. Cells were pre-treated with 10 μM LY294002 (AKT-upstream inhibitor) or U0126 (ERK inhibitor) for 1 h before combined treatment. Cell viability was detected by MTT assay. (c) AKT inhibitor enhances combined treatment-induced PARP cleavage and AKT inactivation. (d) ERK inhibitor enhances combined treatment-induced PARP cleavage and ERK inactivation. All data here are expressed as means±S.D. of triplicates. All images shown here are representative of three independent experiments with similar results. Bars with different characters are statistically different at P<0.05 level

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