Abstract
By laser scanning fluorescence microscopy for quantitative measurement of fluorescence intensity changes on egg surface stained with fluorescein isothiocvanate during cleavage furrow extending forward, it was found that in area of presumptive cleavage furrow the scanning curve became ∨ shape, indicating dark stripe appeared in that place. Then the fluorescence intensity increased at the place where the bottom of ∨ shape had located, and the scanning curve turned to ∧ shape, indicating single stripe was formed. While enhanced fluorescence appeared on the borders of ∧ shape, an M shape curve was found, showing double stripe occurred. During the distance between two borders of M shape increasing from 50 μm to 100 μm, a fluorescence peak came to sight in the middle of the M shape, which being the cleavge furrow bottom. The two lateral sides of furrow bottom with decreasing fluorescence were nascent membrane. At that time the curve became W shape. By the sides of cleavage furrow the the stress folds became conspicous after double stripe stage, showing the stretching of the egg surface being increased. With our31, 33 and others32 reports that polylysine could induce the appearance of nascent membrane and phytohemagglutinins could decrease or prevent the appearance of nascent membrane, we believed the idea of Schroeder25 that increasing mechanical stress could initiate nascent membrane formation and thought that the stress lay to the outsides of cleavage furrow.
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*Dedicated to Prefessor Zhen YAO on the occasion of his 80th birthday.
†This work was supported by the director's foundation of Shanghai Institute of Cell Biology
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Gu, G., Xu, C., Zhang, K. et al. Laser scanning fluorescence microscopic measurement of the movement of cleaving egg surface of Rana Amurensis . Cell Res 5, 9–24 (1995). https://doi.org/10.1038/cr.1995.2
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DOI: https://doi.org/10.1038/cr.1995.2
