Abstract
miR-142 and miR-223 have been identified as hematopoietic specific microRNAs. miR-223 has crucial functions in myeloid lineage development. However, the function of miR-142 remains unclear. In this study, we found that both miR-142 and miR-223 attenuated the proliferation of hematopoietic cells, and that miR-223 up-regulated miR-142 expression through the LMO2-L/-S isoforms and CEBP-β. miR-223 negatively regulated both LMO2-L/-S isoforms and CEBP-β post-transcriptionally, while CEBP-β positively regulated the LMO2-L/-S isoforms and both of the LMO2-L/-S isoforms negatively regulated miR-142. These results reveal a novel miR-223—CEBP-β—LMO2—miR-142 regulatory pathway, which has pivotal functions in hematopoiesis.
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This work was supported by the National Natural Science Foundation of China (Grant No. 30771054).
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( Supplementary information is linked to the online version of the paper on the Cell Research website.)
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Mutation primers (PDF 47 kb)
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Real-time PCR detection primers (PDF 8 kb)
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5′ RACE antisense primers (PDF 8 kb)
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Sun, W., Shen, W., Yang, S. et al. miR-223 and miR-142 attenuate hematopoietic cell proliferation, and miR-223 positively regulates miR-142 through LMO2 isoforms and CEBP-β. Cell Res 20, 1158–1169 (2010). https://doi.org/10.1038/cr.2010.134
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DOI: https://doi.org/10.1038/cr.2010.134
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