Abstract
The ratio of auxin and cytokinin plays a crucial role in regulating aerial architecture by promoting or repressing axillary bud outgrowth. We have previously identified an Arabidopsis mutant bud2 that displays altered root and shoot architecture, which results from the loss-of-function of S-adenosylmethionine decarboxylase 4 (SAMDC4). In this study, we demonstrate that BUD2 could be induced by auxin, and the induction is dependent on auxin signaling. The mutation of BUD2 results in hyposensitivity to auxin and hypersensitivity to cytokinin, which is confirmed by callus induction assays. Our study suggests that polyamines may play their roles in regulating the plant architecture through affecting the homeostasis of cytokinins and sensitivities to auxin and cytokinin.
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Acknowledgements
We thank Dr Ottoline Leyser (University of York, UK) for providing axr1-3 and Dr Mark Estelle (UC, San Diego, USA) for providing tir1afb2afb3 triple mutant seeds. This work was supported by a grant from the National Natural Science Foundation of China (30830009).
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Supplementary information, Figure S1
Auxin contents in bud2 and wild-type plants. (PDF 152 kb)
Supplementary information, Figure S2
Comparison of polar auxin transport (PAT) of inflorescence stems and roots between the wild-type and bud2. (PDF 157 kb)
Supplementary information, Table S1
Primers used for real-time PCR (PDF 121 kb)
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Cui, X., Ge, C., Wang, R. et al. The BUD2 mutation affects plant architecture through altering cytokinin and auxin responses in Arabidopsis. Cell Res 20, 576–586 (2010). https://doi.org/10.1038/cr.2010.51
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DOI: https://doi.org/10.1038/cr.2010.51
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