Abstract
microRNAs (miRNAs) play important roles in the regulation of gene expression. In Arabidopsis, mature miRNAs are processed from primary miRNA transcripts (pri-miRNAs) by nuclear HYL1/SE/DCL1 complexes that form Dicing bodies (D-bodies). Here we report that an RNA-binding protein MOS2 binds to pri-miRNAs and is involved in efficient processing of pri-miRNAs. MOS2 does not interact with HYL1, SE, and DCL1 and is not localized in D-bodies. Interestingly, in the absence of MOS2, the recruitment of pri-miRNAs by HYL1 is greatly reduced and the localization of HYL1 in D-bodies is compromised. These data suggest that MOS2 promotes pri-miRNA processing through facilitating the recruitment of pri-miRNAs by the Dicing complexes.
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Acknowledgements
We thank Dr Anthony Millar (The Australian National University, Australian) for pMIR159b::GUS, Dr Bonnie Bartel (Rice University, USA) for pMIR398c::GUS and Dr H Vaucheret (Institut National de la Recherche Agronomique, France) for the hyl1-2 and ago1-25 mutants. This work was supported in part by China National Funds for Distinguished Young Scientists (31225015) and National Basic Research Program of China (2012CB910900) to Y Q.
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( Supplementary information is linked to the online version of the paper on the Cell Research website.)
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Supplementary information, Table S1
Genes that are downregulated in the mos2-2 mutant (XLS 110 kb)
Supplementary information, Table S2
Genes that are upregulated in the mos2-2 mutant (XLS 42 kb)
Supplementary information, Table S3
Primers and probes (XLS 40 kb)
Supplementary information, Figure S1
Complementation of mos2-2 with p35S::FLAG-MOS2 transgene. (PDF 219 kb)
Supplementary information, Figure S2
Localization of MOS-YFP and HYL1-YFP in the root cells of transgenic plants expressing the YFP fusion proteins under the control of their native promoters. (PDF 273 kb)
Supplementary information, Figure S3
Detection of interactions between DCL1, HYL1, and SE in the Col-0 and mos2-2 cells. (PDF 467 kb)
Supplementary information, Figure S4
Diagrams that show the positions of primers in the pri-miRNA sequences. (PDF 231 kb)
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Wu, X., Shi, Y., Li, J. et al. A role for the RNA-binding protein MOS2 in microRNA maturation in Arabidopsis. Cell Res 23, 645–657 (2013). https://doi.org/10.1038/cr.2013.23
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DOI: https://doi.org/10.1038/cr.2013.23
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