Abstract
Gene expression from both parental genomes is required for completion of embryogenesis. Differential methylation of each parental genome has been observed in mouse and human preimplantation embryos. It is possible that these differences in methylation affect the level of gene transcripts from each parental genome in early developing embryos. The aim of this study was to investigate if there is a parent-specific pattern of BRCA1 expression in human embryos and to examine if this affects embryo development when the embryo carries a BRCA1 or BRCA2 pathogenic mutation. Differential parental expression of ACTB, SNRPN, H19 and BRCA1 was semi-quantitatively analysed by minisequencing in 95 human preimplantation embryos obtained from 15 couples undergoing preimplantation genetic diagnosis. BRCA1 was shown to be differentially expressed favouring the paternal transcript in early developing embryos. Methylation-specific PCR showed a variable methylation profile of BRCA1 promoter region at different stages of embryonic development. Embryos carrying paternally inherited BRCA1 or 2 pathogenic variants were shown to develop more slowly compared with the embryos with maternally inherited BRCA1 or 2 pathogenic mutations. This study suggests that differential demethylation of the parental genomes can influence the early development of preimplantation embryos. Expression of maternal and paternal genes is required for the completion of embryogenesis.
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PT and SSB: substantial contributions to conception and design. PT, AD and PS: acquisition of data. PT and SSB: interpretation of data. PT: analysis and drafting the article. PT, AD, PS and SSB: revising it critically for important intellectual content, and final approval of the version to be published.
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Tulay, P., Doshi, A., Serhal, P. et al. Differential expression of parental alleles of BRCA1 in human preimplantation embryos. Eur J Hum Genet 25, 37–42 (2017). https://doi.org/10.1038/ejhg.2016.121
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DOI: https://doi.org/10.1038/ejhg.2016.121
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