Figure 2 | Experimental & Molecular Medicine

Figure 2

From: Estradiol regulates miR-135b and mismatch repair gene expressions via estrogen receptor-β in colorectal cells

Figure 2

Effect of Estrogen (E2) on inhibiting the expression of miR-31, miR-135b and miR-155. Quantitative RT-PCR analysis indicates that E2 induced the down-regulation of mature miR-31, miR-155, and miR-135b with time, but not miR-223 in COLO205 cells (A). ICI182,780 reversed E2-induced inhibition of miR-31, miR-155 and miR-135b of COLO205 cells (B). SW480 (C) and MCF7 (D) cells were treated with EtOH and E2 for 12 h, there were no significant changes in miR-31, miR-155 and miR-135b expression. Values presented were fold increase compared to EtOH for each miRNA and were calculated as described in 'Materials and Methods' section. Mean ± SD from 3 independent experiments. Significant differences between treated group versus EtOH control were calculated by ANOVA (*P < 0.05 and **P < 0.01).

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