Figure 5
Correlations of serum Estrogen levels with miRNA and ER-β mRNA expression in CRC patients' tissues. Correlations of serum Estrogen levels with miRNA and ER-β mRNA expression in CRC patients' tissues. Serum Estrogen level was measured by ELISA assay whereas miRNA and ER-β mRNA was analyzed by RT-qPCR assay as described in Material and Methods. Correlation of serum estrogen concentrations with miR135b (A) in tumor ΔCt and ΔΔCt (tumor versus non-tumor). Correlation of serum estrogen concentrations with miR-31 (B) in tumor ΔCt and ΔΔCt. Correlation between the expression levels of miR-155 as well as miR-203 (C) in patients' cancer tissues and serum estrogen concentrations. Correlations of serum Estrogen concentrations with ER-β Mrna (D) in tumor ΔCt and ΔΔCt. Box plots with median, 25 th and 75 th percentiles, and range of expression levels of miR-31, miR-155, miR-135b, and miR-203 (E) in 18 tumor tissues relative to adjacent non-tumor tissue. Significant levels of differences were determined by ANOVA. Box plots with median, 25 th and 75 th percentiles, and range of expression levels of ER-β, hMLH1, hMLH2, (F) in 18 tumor tissues relative to adjacent non-tumor tissue. Significant levels of differences were determined by ANOVA (*P < 0.05 and **P < 0.01).
