Abstract
Purpose
To develop a non-invasive method for quantification of blood and pigment distributions across the posterior pole of the fundus from multispectral images using a computer-generated reflectance model of the fundus.
Methods
A computer model was developed to simulate light interaction with the fundus at different wavelengths. The distribution of macular pigment (MP) and retinal haemoglobins in the fundus was obtained by comparing the model predictions with multispectral image data at each pixel. Fundus images were acquired from 16 healthy subjects from various ethnic backgrounds and parametric maps showing the distribution of MP and of retinal haemoglobins throughout the posterior pole were computed.
Results
The relative distributions of MP and retinal haemoglobins in the subjects were successfully derived from multispectral images acquired at wavelengths 507, 525, 552, 585, 596, and 611 nm, providing certain conditions were met and eye movement between exposures was minimal. Recovery of other fundus pigments was not feasible and further development of the imaging technique and refinement of the software are necessary to understand the full potential of multispectral retinal image analysis.
Conclusion
The distributions of MP and retinal haemoglobins obtained in this preliminary investigation are in good agreement with published data on normal subjects. The ongoing development of the imaging system should allow for absolute parameter values to be computed. A further study will investigate subjects with known pathologies to determine the effectiveness of the method as a screening and diagnostic tool.
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Acknowledgements
We gratefully acknowledge financial support from the Engineering and Physical Sciences Research Council (EPSRC) under Grant number GR/S09906/01 and from the Advantage West Midlands for funding of the hyperspectral imaging facilities under grant number BGIN3264. We thank: Mr James Osborne for his generous donation of the Zeiss RCM250 fundus camera used in this work; Mr Peter Lundh of the Institute of Ophthalmology provided technical help with the design of the imaging system; the Optometry Department of the Birmingham and Midland Eye Centre for kindly loaning an artificial eye and lenses; the Consultants and medical staff of the Birmingham and Midland Eye Centre for assisting with the selection of suitable subjects.
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The preliminary results of this research were presented at the 2005 EVER Congress.
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Calcagni, A., Gibson, J., Styles, I. et al. Multispectral retinal image analysis: a novel non-invasive tool for retinal imaging. Eye 25, 1562–1569 (2011). https://doi.org/10.1038/eye.2011.202
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DOI: https://doi.org/10.1038/eye.2011.202
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