Table 1 Sequences of primer pairs used for quantitative RT-PCR analysis, MassARRAY and (h)MeDIP
Name | Use | Primer (5 ′–3′)a |
|---|---|---|
Nono | F: TGCTCCTGTGCCACCTGGTACTC | |
R: CCGGAGCTGGACGGTTGAATGC | ||
Pparγ | F: CCCTGGCAAAGCATTTGTAT | |
R: GAAACTGGCACCCTTGAAAA | ||
Cebpα | RT-PCR | F: TGTGCGAGCACGAGACGTC |
R: AACTCGTCGTTGAAGGCGG | ||
Fabp4 | F: TTCGATGAAATCACCGCAGA | |
R: AGGGCCCCGCCATCT | ||
Cebpβ | F: GCAAGAGCCGCGACAAG | |
R: GGCTCGGGCAGCTGCTT | ||
Pparγ | Bisulfite-treated PCR | F: aggaagagagTTTTGTTTTTATTTGTTTGTTTTGG |
R: cagtaatacgactcactatagggagaaggctACTTATTTTTATCTCAAATCATCAACAAT | ||
Cebpα | F: aggaagagagGATTTTATGGGGGAGTTAGAGTTTT | |
R: cagtaatacgactcactatagggagaaggctCCCAACAAACACCATCCTACTAAC | ||
Fabp4 | F: aggaagagagTTTTAATGTTAAGGTTTAAGAATGTGTGA | |
R: cagtaatacgactcactatagggagaaggctAACAAACAACCAAAAATTCCCACTA | ||
Pparγ | (h)MeDIP | F: CCTGAATTTACCCGAGCTGA |
R: TTATCTCGGAGGCGGTAAGA |
