Abstract
White-rot fungi are major degraders of woody materials in terrestrial environments because of their ability to decompose lignin. However, little is known on the possible associations of white-rot fungi with other microorganisms during wood decay. We investigated the numbers, community composition and functional traits of bacteria present in natural wood samples under advanced decay by the white-rot basidiomycete Hypholoma fasciculare. The wood samples contained high numbers of cultivable bacteria (0.2–8 × 109 colony forming units (CFU) per g of dry wood). Most cultivable bacteria belonged to Proteobacteria and Acidobacteria (75% and 23% of sequences, respectively). The same phyla were also found to be dominant (59% and 23%, respectively) using a non-culturable quantification technique, namely, direct cloning and sequencing of 16sRNA genes extracted from wood. Bacteria that could be subcultured consisted of acid-tolerant strains that seemed to rely on substrates released by lignocellulolytic enzyme activities of the fungus. There were no indications for antagonism (antibiosis) of the bacteria against the fungus.
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Acknowledgements
This work was supported by the Ministry of Education, Youth and Sports of the Czech Republic (LC06066) and by the Institutional Research Concept No. AV0Z50200510 of the Institute of Microbiology of the ASCR, v.v.i. The Federation of European Microbiological Societies is acknowledged for a travel grant to Vendula Valášková. The authors also thank Dr Miroslav Kolařík for valuable discussion about data analysis.
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Valášková, V., de Boer, W., Klein Gunnewiek, P. et al. Phylogenetic composition and properties of bacteria coexisting with the fungus Hypholoma fasciculare in decaying wood. ISME J 3, 1218–1221 (2009). https://doi.org/10.1038/ismej.2009.64
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DOI: https://doi.org/10.1038/ismej.2009.64
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