Abstract
N2O is a potent greenhouse gas involved in the destruction of the protective ozone layer in the stratosphere and contributing to global warming. The ecological processes regulating its emissions from soil are still poorly understood. Here, we show that the presence of arbuscular mycorrhizal fungi (AMF), a dominant group of soil fungi, which form symbiotic associations with the majority of land plants and which influence a range of important ecosystem functions, can induce a reduction in N2O emissions from soil. To test for a functional relationship between AMF and N2O emissions, we manipulated the abundance of AMF in two independent greenhouse experiments using two different approaches (sterilized and re-inoculated soil and non-mycorrhizal tomato mutants) and two different soils. N2O emissions were increased by 42 and 33% in microcosms with reduced AMF abundance compared to microcosms with a well-established AMF community, suggesting that AMF regulate N2O emissions. This could partly be explained by increased N immobilization into microbial or plant biomass, reduced concentrations of mineral soil N as a substrate for N2O emission and altered water relations. Moreover, the abundance of key genes responsible for N2O production (nirK) was negatively and for N2O consumption (nosZ) positively correlated to AMF abundance, indicating that the regulation of N2O emissions is transmitted by AMF-induced changes in the soil microbial community. Our results suggest that the disruption of the AMF symbiosis through intensification of agricultural practices may further contribute to increased N2O emissions.
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Acknowledgements
We would like to thank Yoram Kapulnik for kindly providing seeds of the BC1-mutant/wildtype tomato pair, Caroline Scherrer, Philipp Weber and Christoph Barendregt for helping with the gas measurements, Bernhard Schmid, Franz Conen and Cameron Wagg for helpful discussions in statistics, and Tim George, the editor and two anonymous reviewers for useful comments on the manuscript. This study was funded by the Swiss National Science Foundation (SNSF), grant no. 125428 awarded to MvdH.
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Bender, S., Plantenga, F., Neftel, A. et al. Symbiotic relationships between soil fungi and plants reduce N2O emissions from soil. ISME J 8, 1336–1345 (2014). https://doi.org/10.1038/ismej.2013.224
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DOI: https://doi.org/10.1038/ismej.2013.224
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