Abstract
The guts of metazoans are in permanent contact with the microbial realm that includes beneficial symbionts, nonsymbionts, food-borne microbes and life-threatening pathogens. However, little is known concerning how host immunity affects gut bacterial community. Here, we analyze the role of a dual oxidase gene (BdDuox) in regulating the intestinal bacterial community homeostasis of the oriental fruit fly Bactrocera dorsalis. The results showed that knockdown of BdDuox led to an increased bacterial load, and to a decrease in the relative abundance of Enterobacteriaceae and Leuconostocaceae bacterial symbionts in the gut. The resulting dysbiosis, in turn, stimulates an immune response by activating BdDuox and promoting reactive oxygen species (ROS) production that regulates the composition and structure of the gut bacterial community to normal status by repressing the overgrowth of minor pathobionts. Our results suggest that BdDuox plays a pivotal role in regulating the homeostasis of the gut bacterial community in B. dorsalis.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (No. 31572008), the earmarked fund for the China Agricultural Research System (Nos. CARS-27), International Atomic Energy Agency’s Coordinated Research Project (No. D42016) and Fundamental Research Funds for the Central Universities (No. 2014PY005 and 2662015PY129). We thank Zhili He from Institute for Environmental Genomics, University of Oklahoma, for his insightful advice and comments that improved the manuscript.
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Yao, Z., Wang, A., Li, Y. et al. The dual oxidase gene BdDuox regulates the intestinal bacterial community homeostasis of Bactrocera dorsalis. ISME J 10, 1037–1050 (2016). https://doi.org/10.1038/ismej.2015.202
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DOI: https://doi.org/10.1038/ismej.2015.202
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