Abstract
Despite accounting for the majority of sedimentary methane, the physiology and relative abundance of subsurface methanogens remain poorly understood. We combined intact polar lipid and metagenome techniques to better constrain the presence and functions of methanogens within the highly reducing, organic-rich sediments of Antarctica’s Adélie Basin. The assembly of metagenomic sequence data identified phylogenic and functional marker genes of methanogens and generated the first Methanosaeta sp. genome from a deep subsurface sedimentary environment. Based on structural and isotopic measurements, glycerol dialkyl glycerol tetraethers with diglycosyl phosphatidylglycerol head groups were classified as biomarkers for active methanogens. The stable carbon isotope (δ13C) values of these biomarkers and the Methanosaeta partial genome suggest that these organisms are acetoclastic methanogens and represent a relatively small (0.2%) but active population. Metagenomic and lipid analyses suggest that Thaumarchaeota and heterotrophic bacteria co-exist with Methanosaeta and together contribute to increasing concentrations and δ13C values of dissolved inorganic carbon with depth. This study presents the first functional insights of deep subsurface Methanosaeta organisms and highlights their role in methane production and overall carbon cycling within sedimentary environments.
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Acknowledgements
We thank the shipboard party of IODP Expedition 318 for supporting this work, especially co-chiefs Escutia and Brinkhuis, Staff Scientist Klaus and geochemistry team: James Bendle, Tina van de Flierdt, and Francisco Jimenez-Espejo. We thank Charles Pepe-Ranney and Benjamin Tully for bioinformatics assistance, David Mucciarone for isotope geochemistry assistance, Kai-Uwe Hinrichs and John Spear for the use of their facilities, Julius Lipp for provision of IPL standards and Beth Orcutt for manuscript comments. The Deep Carbon Observatory’s Census of Deep Life project funded by the Alfred P Sloan Foundation provided sequencing services. Carr was funded by the US National Science Foundation (NSF OCE-0939564) and the Center for Dark Energy Biosphere Investigations (funded by NSF OCE-1521614, publication no. 390). FS and RES acknowledge funding from NASA Astrobiology grant (NNA13AA90A) and the Central Research Development Fund, University of Bremen. Any use of trade, firm or product names is for descriptive purposes only and does not imply endorsement by the US Government.
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Carr, S., Schubotz, F., Dunbar, R. et al. Acetoclastic Methanosaeta are dominant methanogens in organic-rich Antarctic marine sediments. ISME J 12, 330–342 (2018). https://doi.org/10.1038/ismej.2017.150
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DOI: https://doi.org/10.1038/ismej.2017.150
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