Abstract
Streptomycetes are the major natural source of clinical antibiotics. The enhanced secondary metabolite production of many streptomycetes by S-adenosylmethionine (SAM) in previous studies suggested the existence of a common SAM regulatory effect. We screened nine proteins using the phosphoprotein purification column from Streptomyces coelicolor. Among them, genes (SCO5477, SCO5113, SCO4647, SCO4885 and SCO1793) for five proteins were disrupted by insertion mutation. The undecylprodigiosin and actinorhodin productions were changed in all mutations. The SAM-induced enhancement of actinorhodin production was abolished by all mutations except SCO4885 mutation, which reduced the production of actinorhodin and undecylprodigiosin with SAM treatment. This study demonstrates that phosphoprotein affinity purification can be used as a screening method to identify the proteins involved SAM signaling.
Similar content being viewed by others
Log in or create a free account to read this content
Gain free access to this article, as well as selected content from this journal and more on nature.com
or
References
Huang, J. et al. Cross-regulation among disparate antibiotic biosynthetic pathways of Streptomyces coelicolor. Mol. Microbiol. 58, 1276–1287 (2005).
Novotna, J. et al. Proteomic studies of diauxic lag in the differentiating prokaryote Streptomyces coelicolor reveal a regulatory network of stress-induced proteins and central metabolic enzymes. Mol. Microbiol. 48, 1289–1303 (2003).
Kim, D. J. et al. Accumulation of S-adenosyl-L-methionine enhances production of actinorhodin but inhibits sporulation in Streptomyces lividans TK23. J. Bacteriol. 185, 592–600 (2003).
Okamoto, S., Lezhava, A., Hosaka, T., Okamoto-Hosoya, Y. & Ochi, K. Enhanced expression of S-adenosylmethionine synthetase causes overproduction of actinorhodin in Streptomyces coelicolor A3(2). J. Bacteriol. 185, 601–609 (2003).
Shin, S.K., Xu, D., Kwon, H. J. & Suh, J. W. S-adenosylmethionine activates adpA transcription and promotes streptomycin biosynthesis in Streptomyces griseus. FEMS Microbiol. Lett. 259, 53–59 (2006).
Huh, J. H. et al. Widespread activation of antibiotic biosynthesis by S-adenosylmethionine in streptomycetes. FEMS Microbiol. Lett. 238, 439–447 (2004).
Kieser, T., Bibb, M. J., Buttner, M. J., Chater, K. F. & Hopwood, D. A. Practical Streptomyces genetics (John Innes Foundation, Norwich, United Kingdom, 2000).
Hopwood, D. A. et al. Genetic Manipulation of Streptomyces. A Laboratory Manual (John Innes Foundation, Norwich, 1985).
Li, M., Kim, T. J., Kwon, H. J. & Suh, J. W. Effects of extracellular ATP on the physiology of Streptomyces coelicolor A3(2). FEMS Microbiol. Lett. 286, 24–31 (2008).
Park, S. S. et al. Mass spectrometric screening of transcriptional regulators involved in antibiotic biosynthesis in Streptomyces coelicolor A3(2). J. Ind. Microbiol. Biotechnol. 36, 1073–1083 (2009).
D’Alia, D., Nieselt, K., Steigele, S., Muller, J., Verburg, I. & Takano, E. Noncoding RNA of glutamine synthetase I modulates antibiotic production in Streptomyces coelicolor A3(2). J. Bacteriol. 192, 1160–1164 (2010).
Park, H. S., Shin, S. K., Yang, Y. Y., Kwon, H. J. & Suh, J. W. Accumulation of S-adenosylmethionine induced oligopeptide transporters including BldK to regulate differentiation events in Streptomyces coelicolor M145. FEMS Microbiol. Lett. 249, 199–206 (2005).
Acknowledgements
This work was supported by a grant (11-2008-16-001-00) from the 21C Frontier Microbial Genomics and Application Center program, the Korean Ministry of Science and Technology and by a National Research Foundation of Korea (NRF) grant, funded by the Korean government (MEST) (Grant#: 2009-0071562). Lingzhu Meng is supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD) (KRF-2007-211-C00039) and the second stage of the Brain Korea 21 (BK21) Project.
Author information
Authors and Affiliations
Corresponding authors
Additional information
Dedicated to late Dr C Richard Hutchinson for his exceptional contributions to natural product biosynthesis, engineering and drug discovery.
Supplementary Information accompanies the paper on The Journal of Antibiotics website
Supplementary information
Rights and permissions
About this article
Cite this article
Meng, L., Yang, S., Palaniyandi, S. et al. Phosphoprotein affinity purification identifies proteins involved in S-adenosyl-L-methionine-induced enhancement of antibiotic production in Streptomyces coelicolor. J Antibiot 64, 97–101 (2011). https://doi.org/10.1038/ja.2010.148
Received:
Revised:
Accepted:
Published:
Issue date:
DOI: https://doi.org/10.1038/ja.2010.148
Keywords
This article is cited by
-
Comparative proteomic and metabolomic analysis of Streptomyces tsukubaensis reveals the metabolic mechanism of FK506 overproduction by feeding soybean oil
Applied Microbiology and Biotechnology (2017)
-
Tryptophan promotes morphological and physiological differentiation in Streptomyces coelicolor
Applied Microbiology and Biotechnology (2015)
-
An ABC transporter complex containing S-adenosylmethionine (SAM)-induced ATP-binding protein is involved in antibiotics production and SAM signaling in Streptomyces coelicolor M145
Biotechnology Letters (2012)
-
Investigation of proteomic responses of Streptomyces lydicus to pitching ratios for improving streptolydigin production
Biotechnology and Bioprocess Engineering (2012)
