Abstract
A novel Gram-stain-positive actinobacterium, designated RI148-Li105T, was isolated from a lichen sample from Rishiri Island, Japan, and its taxonomic position was investigated by a polyphasic approach. 16S rRNA gene sequencing study indicated that strain RI148-Li105T was related to the type strain of Luteimicrobium subarcticum, with a similarity of 97.8%. Cells of strain RI148-Li105T exhibited a rod–coccus cycle. The diagnostic cell-wall diamino acid of this organism was lysine and the peptidoglycan type was found to be A4α. The predominant menaquinones were MK-8(H2) and MK-9(H2), and the major fatty acids were iso-C16:0, C17:1 ω9c and C17:0. The DNA G+C content was 73.6 mol%. The major phenotypic characteristics of strain RI148-Li105T basically corresponded to those of the genus Luteimicrobium excluding the fatty acid composition. These results suggest that strain RI148-Li105T should be affiliated with the genus Luteimicrobium. Meanwhile, DNA–DNA hybridization and some phenotypic characteristics revealed that the strain differs from L. subarcticum. Therefore, strain RI148-Li105T represents a novel species of the genus Luteimicrobium, for which the name Luteimicrobium album sp. nov. is proposed. The type strain of Luteimicrobium album is RI148-Li105T (=NBRC 106348T=DSM 24866T).
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Acknowledgements
We are grateful to Mr Yuya Sakuraki for excellent technical assistance. This study was partly supported by a research grant from the Institute for Fermentation, Osaka (IFO), Japan.
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Hamada, M., Yamamura, H., Komukai, C. et al. Luteimicrobium album sp. nov., a novel actinobacterium isolated from a lichen collected in Japan, and emended description of the genus Luteimicrobium. J Antibiot 65, 427–431 (2012). https://doi.org/10.1038/ja.2012.45
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DOI: https://doi.org/10.1038/ja.2012.45
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