Abstract
The motile actinomycete strain RI50-RCA114T was isolated using rehydration and centrifugation method from a soil sample obtained from Rishiri Island in Japan. The taxonomic status of this organism was established using a polyphasic approach. Cells of strain RI50-RCA114T were Gram positive, aerobic, motile and formed irregular sporangia. The strain grew in the presence of 0–2% (w/v) NaCl, between pH 5 and 8, and over a temperature range of 20–37°C, with optimal growth at 30°C. Whole-cell hydrolysates of the strain contained meso-diaminopimelic acid, galactose, glucose and mannose, in addition to one unidentified O-methyl-hexose. The predominant menaquinone was MK-9(H4), and the major polar lipids comprised phosphatidylethanolamine, diphosphatidylglycerol and phosphatidyl-N-methylethylethanolamine. The major cellular fatty acids were iso-C16:0, iso-C15:0 and anteiso-C17:0. Comparative 16S ribosomal RNA gene sequence analysis revealed that strain RI50-RCA114T had the closest sequence similarity with Actinoplanes globisporus JCM 3186T (97.6%). However, DNA–DNA hybridization assays as well as physiological and biochemical analyses differentiated strain RI50-RCA114T from its closest phylogenetic relative. On the basis of these data, we propose that strain RI50-RCA114T (=NBRC 108556T=BCC 49184T) be classified as the type strain of a novel Actinoplanes species and named Actinoplanes rishiriensis sp. nov.
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Acknowledgements
This study was supported in part by a research grant from the Institute for Fermentation, Osaka (IFO), Japan. We are grateful to Dr Jean P Euzéby for support with nomenclature.
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Yamamura, H., Shimizu, A., Nakagawa, Y. et al. Actinoplanes rishiriensis sp. nov., a novel motile actinomycete isolated by rehydration and centrifugation method. J Antibiot 65, 249–253 (2012). https://doi.org/10.1038/ja.2012.8
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DOI: https://doi.org/10.1038/ja.2012.8
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