Abstract
A polyphasic approach was used to determine the taxonomic position of actinomycete strain R1-NS-10T, which was isolated from a sample of strawberry root rhizosphere obtained from Hokuto, Yamanashi, Japan. Strain R1-NS-10T was Gram-staining-positive and aerobic, and formed brownish-white aerial mycelia and grayish-brown substrate mycelia on ISP-2 medium. The strain grew in the presence of 0–5% (w/v) NaCl and optimally grew without NaCl. The strain grew at pH 5–8, and the optimum for growth was pH 7. The optimal growth temperature was 30 °C, but the strain grew at 5–37 °C. Whole-cell hydrolysates of strain R1-NS-10T contained A2pm, galactose, mannose and rhamnose. The predominant menaquinones were MK-9(H6) and MK-9(H8). The major cellular fatty acids were anteiso-C15:0 and iso-C16:0. Comparative 16S rRNA gene sequence analysis revealed that strain R1-NS-10T was most closely related to Streptomyces prunicolor NBRC 13075T (99.4%). The draft genome sequences of both strains were determined for characterization of genome sequence-related parameters such as average nucleotide identity (ANI) and the diversity of secondary metabolite biosynthetic gene clusters. DNA–DNA hybridization (DDH) and ANI values for both strains were below the species delineation cutoff, and differences in physiological and biochemical characteristics differentiated strain R1-NS-10T from its closest phylogenetic relative. On the basis of these data, we propose that strain R1-NS-10T (=NBRC 108812T=KCTC 29186T) should be classified as the type strain of a novel Streptomyces species named Streptomyces hokutonensis sp. nov.
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Acknowledgements
This work was conducted and supported under the joint research project ‘Locally produced and Consumed’ between the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Hokuto City and the University of Yamanashi. This study was supported in part by a research grant from the Institute for Fermentation, Osaka (IFO), Japan. We are grateful to Dr Jean P Euzéby for assistance with nomenclature.
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Yamamura, H., Ashizawa, H., Hamada, M. et al. Streptomyces hokutonensis sp. nov., a novel actinomycete isolated from the strawberry root rhizosphere. J Antibiot 67, 465–470 (2014). https://doi.org/10.1038/ja.2014.20
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DOI: https://doi.org/10.1038/ja.2014.20
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