Abstract
Chromatographic separation of a crude extract obtained from a fermentation broth of a chemically unknown bacterium Shewanella piezotolerans WP3 collected in deep-sea yielded three new indole alkaloids namely shewanellines A (1a), B (1b) and C (2), together with 12 known indole alkaloids. The structures were unambiguously elucidated on the basis of 1D and 2D NMR (1H, 13C, COSY, HMBC, HSQC and NOESY) in association with MS and CD data. Compounds 1–4, 7, 9 and 11–14 were selected for the evaluation of their cytotoxic activities against human tumor cell lines HL-60 and BEL-7402, whereas compounds 2, 4 and 9 exhibited significant inhibition toward HL-60.
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Acknowledgements
This work was supported by grants from the NSFC (No.30930109), COMRA (DY125-15-T-01) and SOA (2010319123366025-4), and MOST 863 Project (2011AA090701, 2010DFA31610).
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Wang, Y., Tang, X., Shao, Z. et al. Indole-based alkaloids from deep-sea bacterium Shewanella piezotolerans with antitumor activities. J Antibiot 67, 395–399 (2014). https://doi.org/10.1038/ja.2014.3
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DOI: https://doi.org/10.1038/ja.2014.3
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