Abstract
A rapid and simple method was developed and validated for the determination of colistin A and B in Mueller–Hinton broth using LC-tandem MS. Both analyte and internal standard (IS) (polymyxin B1) were determined using ESI. The MS data were obtained via the selected reaction monitoring in positive-ion mode. A linear regression (weighted 1/concentration) was used to fit calibration curves over the concentration range of 0.0241–2.41 μg ml−1 for colistin A and 0.0439–4.39 μg ml−1 for colistin B. No interference peaks were found in the blank Mueller–Hinton broth tested. Inter- and intraday precision and accuracy were within 85–115% (coefficient of variation). Colistin was stable in the autosampler for at least 24 h at 4 °C and in Mueller–Hinton broth for at least 120 h at 35 °C. This assay has been successfully used to determine colistin A and B in Mueller–Hinton broth for in vitro pharmacodynamic model studies. Accurate determination of colistin in bacterial growth medium has a vital role in the studies examining dosage regimen of and bacterial resistance to colistin.
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Acknowledgements
This study was supported by the New Drug Creation and Manufacturing Program of the Ministry of Science and Technology of China (2012ZX09303004-001) and the National Natural Science Foundation of China (81373493).
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Zhao, M., Cao, YR., Guo, BN. et al. LC-MS/MS determination of colistin in Mueller–Hinton broth for in vitro pharmacodynamic studies. J Antibiot 67, 825–829 (2014). https://doi.org/10.1038/ja.2014.73
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DOI: https://doi.org/10.1038/ja.2014.73
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