Abstract
A novel Gram-stain-positive actinomycete, designated MB-PO13T, was isolated from a tunicate (Molgula manhattensis) collected in Tokyo Bay, Japan, and its taxonomic position was studied by a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain MB-PO13T was closely related to Streptomyces graminisoli JR-12T (99.72% 16S rRNA gene sequence similarity) and Streptomyces shenzhenensis 172115T (99.23%). The strain contained LL-diaminopimelic acid in the whole-cell hydrolysate. The predominant menaquinones were MK-9(H8) and MK-9(H6) and the major fatty acids were anteiso-C15:0, iso-C16:0, iso-C14:0 and C16:0. These data supported the affiliation of the novel strain to the genus Streptomyces. Meanwhile, results of DNA−DNA hybridization and physiological and biochemical tests indicated that strain MB-PO13T was distinguished from known Streptomyces type strains. Therefore, strain MB-PO13T represents a novel species of the genus Streptomyces for which the name Streptomyces hyaluromycini sp. nov. is proposed; the type strain is MB-PO13T (=NBRC 110483T =DSM 100105T).
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Acknowledgements
The authors are grateful to Dr Ken-ichiro Suzuki (NBRC) and NBRC staff for their assistance for chemotaxonomic study. This work was supported by the Japan Society for the Promotion of Science (JSPS) for Young Scientists (15K18692) and Institute for Fermentation, Osaka (IFO) for Young Scientists.
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Harunari, E., Hamada, M., Shibata, C. et al. Streptomyces hyaluromycini sp. nov., isolated from a tunicate (Molgula manhattensis). J Antibiot 69, 159–163 (2016). https://doi.org/10.1038/ja.2015.110
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DOI: https://doi.org/10.1038/ja.2015.110
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