Coculnol, a new penicillic acid produced by a coculture of Fusarium solani FKI-6853 and Talaromyces sp. FKA-65

Fungal strains FKI-6853 and FKA-65 were isolated from the soil samples collected from Haha-jima, Bonin Islands, Tokyo, Japan and Kouzu island, Izu Islands, Tokyo, Japan, respectively. The internal transcribed spacer (ITS) sequences of the strains FKI-6853 and FKA-65 were decided and deposited at the DNA Data Bank of Japan with accession numbers LC002783 and LC002784, respectively. The ITS region of FKI-6853 and FKA-65 were compared with the sequences in the GenBank database by BLASTN 2.2.29 analysis.³ FKI-6853 showed a 99.5% similarity with FMR 7141 (F. solani, GenBank accession number AM412637), and FKA-65 showed a 99.4% similarity with CBS 133147 (ex-type of Talaromyces thailandensis,⁴ GenBank accession number JN898041). From the results of sequencing analyses, the producing strains FKI-6853 and FKA-65 were identified as F. solani and Talaromyces sp., respectively.

Strains FKI-6853 and FKA-65 were grown and maintained on a Miura's medium (LcA) slant consisting of 0.1% glycerol, 0.08% KH₂PO₄, 0.02% K₂HPO₄, 0.02% MgSO₄·7H₂O, 0.02% KCl, 0.2% NaNO₃, 0.02% yeast extract (Oriental Yeast Co., Ltd., Tokyo, Japan) and 1.5% agar (adjusted to pH 6.0 before sterilization). A loop of spores of F. solani FKI-6853 was inoculated into 10 ml of seed medium, consisting of 2.0% glucose, 0.2% yeast extract, 0.5% Polypepton (Nihon Pharmaceutical Co., Ltd., Tokyo, Japan), 0.05% MgSO₄·7H₂O, 0.1% KH₂PO₄ and 0.1% agar (adjusted to pH 6.0 before sterilization), in a test tube. The seed culture of Talaromyces sp. FKA-65 was also prepared in the same manner. The inoculated tubes were incubated on a reciprocal shaker (300 r.p.m.) at 27 °C for 3 days.

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