Abstract
Chloromonilicin was isolated for the first time from Alternaria sonchi, a mycoherbicide proposed for the control of the noxious weed Sonchus arvensis. The already known alternethanoxins A and B and the three recently isolated phytotoxic polycyclic ethanones named alternethanoxins C–E were also isolated from the same source. Chloromonilicin was identified by spectroscopic data (essentially one-dimensional NMR, 2-dimensional NMR and high-resolution ESI-MS) and its structure was confirmed by single X-ray analysis, which also allowed the assignment of the absolute configuration. This latter was independently confirmed by electronic CD calculations. When chloromonilicin was tested for its antimicrobial activity, it was active at concentrations 0.5–1 μg per disc against four bacterial species and a yeast fungus. The compound inhibited conidial germination of four plant pathogens at concentration of 1–10 μg ml−1. No phytotoxic activity of this antibiotic by leaf-disc puncture bioassay was detected.
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Acknowledgements
We thank Mr L Chistyi and Dr V Zakharov (Research Institute of Hygiene, Occupational Pathology and Human Ecology, Federal Medical Biological Agency, St Petersburg, Russian Federation) for NMR and Dr A Rudenko (Institute of Toxicology, Federal Medical Biology Agency, Saint-Petersburg, Russian Federation) for HR ESIMS spectra. The research work of the Russian group on production, isolation and biological characterization of bioactive metabolites produced by plant pathogens was supported by Russian Fund of Basic Research (project N 12-04-00853). This research was carried out in part in the frame of Programme STAR, financially supported by UniNA and Compagnia di San Paolo. AE is associated with the Istituto di Chimica Biomolecolare del CNR, Pozzuoli, Italy.
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Cimmino, A., Pescitelli, G., Berestetskiy, A. et al. Biological evaluation and determination of the absolute configuration of chloromonilicin, a strong antimicrobial metabolite isolated from Alternaria sonchi. J Antibiot 69, 9–14 (2016). https://doi.org/10.1038/ja.2015.74
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DOI: https://doi.org/10.1038/ja.2015.74
