Abstract
An actinomycete strain, designated MM04-1133T, was isolated from an anthill soil sample collected in Bagan, Myanmar. To establish the taxonomic status of this strain, the isolate was subjected to a polyphasic approach. Strain MM04-1133T was Gram-staining positive, aerobic, motile and formed long and narrow sporangia directly above the surface of the substrate mycelium. Whole-cell hydrolysates of the strain contained 3-OH-diaminopimelic acid, arabinose, glucose, galactose, mannose, rhamnose and xylose. The predominant menaquinones were MK-10(H6) and MK-10(H8). The major cellular fatty acids were iso-C16:0 and anteiso-C17:0. The diagnostic phospholipid was phosphatidylethanolamine. The G+C content of the DNA was 69.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain MM04-1133T clustered within the genus Virgisporangium, with the sequence exhibiting highest similarity (98.5% identity) with Virgisporangium ochraceum NBRC 16418T. The strain grew in the presence of 0–1% (w/v) NaCl, at pH 5–8 and at 20–40 °C, with optimal growth at 30–37 °C. Based on phylogenetic analysis and chemotaxonomic and phenotypic data, we propose classifying this isolate as a novel species of the genus Virgisporangium, to be designated as Virgisporangium myanmarense sp. nov. The type strain is MM04-1133T (=NBRC 112733T=VTCC 910008T).
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Acknowledgements
We are grateful to Dr Bernhard Schink (University of Konstanz, Konstanz, Germany) for assistance with the nomenclature. This study was supported in part by a research grant from the Institute for Fermentation, Osaka (IFO), Japan, and JSPS KAKENHI Grant Number JP26304006. This work was conducted under a Joint Research Program on Conservation and Sustainable Use of Biological Resource between the Department of Biotechnology, National Institute of Technology and Evaluation, Japan, and the Pathein University, representing Ministry of Education, Myanmar.
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Yamamura, H., Miyazaki, S., Ikoma, K. et al. Virgisporangium myanmarense sp. nov., a novel motile actinomycete isolated from an anthill soil in Myanmar. J Antibiot 70, 995–999 (2017). https://doi.org/10.1038/ja.2017.81
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DOI: https://doi.org/10.1038/ja.2017.81
