Table 2 Primer sequences and PCR conditions
Target | Sequence | Product size (bp) | PCR cycles | Annealing temperature (°C) |
---|---|---|---|---|
CXCL1 | F: 5′-CTGCGCCCAAACCGAAGTCA-3′ | 520 | 27 | 55 |
R: 5′-ATCCGCCAGCCTCTATCACAG-3′ | ||||
CXCL8 | F: 5′-TGACTTCCAAGCTGGCCGTG-3′ | 270 | 26 | 55 |
R: 5′-TCTTCACAACCCTCTGCACC-3′ | ||||
G-CSF | F: 5′-GTGCAGGAAGCCACCCCCCTGGGC-3′ | 471 | 34 | 67 |
R: 5′-CCCTCCTGCCCGGCCCTGGAAAGC-3′ | ||||
β-Actin | F: 5′-GGAGCAATGATCTTGATCTT-3′ | 204 | 30 | 55 |
R: 5′-CCTTCCTGGGCATGGAGTCCT-3′ |