Table 2 Primer sequences and PCR conditions

From: Human peritoneal fibroblasts are a potent source of neutrophil-targeting cytokines: a key role of IL-1β stimulation

Target

Sequence

Product size (bp)

PCR cycles

Annealing temperature (°C)

CXCL1

F: 5′-CTGCGCCCAAACCGAAGTCA-3′

520

27

55

 

R: 5′-ATCCGCCAGCCTCTATCACAG-3′

   

CXCL8

F: 5′-TGACTTCCAAGCTGGCCGTG-3′

270

26

55

 

R: 5′-TCTTCACAACCCTCTGCACC-3′

   

G-CSF

F: 5′-GTGCAGGAAGCCACCCCCCTGGGC-3′

471

34

67

 

R: 5′-CCCTCCTGCCCGGCCCTGGAAAGC-3′

   

β-Actin

F: 5′-GGAGCAATGATCTTGATCTT-3′

204

30

55

 

R: 5′-CCTTCCTGGGCATGGAGTCCT-3′

   
  1. F, forward; R, reverse; bp, base pairs.