Table 3 Systemic plasma alanine aminotransferase (ALT) and creatinine (Cr) levels in mice subjected to sham operation, bilateral nephrectomy (BNx) or 30 min renal ischemia reperfusion (RIR)

From: Cytokines induce small intestine and liver injury after renal ischemia or nephrectomy

  

ALT (U/L)

Cr (mg/dl)

  

5 h

24 h

5 h

24 h

C57

Sham

53±6 (4)

61±8 (4)

0.48±0.02 (4)

0.50±0.03 (4)

C57 (WT)

BNx

221±9 (8)*

132±18 (6)*

1.22±0.04 (8)*

2.68±0.13 (6)*

TNF-α KO

BNx

134±11 (4)*#

ND

1.51±0.10 (4)*

ND

IL-6 KO

BNx

82±4 (4)*#

ND

1.17±0.04 (4)*

ND

IL-17A KO

BNx

103±15 (7)*#

128±15 (4)*

1.31±0.03 (7)*

2.45±0.04 (4)*

IL-17R KO

BNx

76±3 (4)*#

ND

1.13±0.05 (4)*

ND

C57 (WT)

RIR

170±12 (8)*

238±18 (8)*

1.13±0.05 (8)*

2.36±0.06 (8)*

TNF-α KO

RIR

95±6 (4)*#

83±10 (4)#

0.68±0.04 (4)#

1.07±0.02 (4)*#

IL-6 KO

RIR

90±6 (4)*#

ND

0.51±0.04 (4)#

ND

IL-17A KO

RIR

104±8 (6)*#

89±8 (4)#

0.66±0.04 (6)#

1.06±0.08 (4)*#

IL-17R KO

RIR

90±6 (4)*#

ND

0.54±0.04 (4)#

ND

IgG Ab

BNx

423±105 (4)*

463±83 (4)*

1.23±0.06 (4)*

2.37±0.12 (4)*

TNF-α Ab

BNx

74±10 (4)+

93±9 (4)*+

0.93±0.05 (4)*

1.91±0.08 (4)*

IL-6 Ab

BNx

87±8 (4)*+

104±5 (4)*+

0.93±0.07 (4)*

2.00±0.16 (4)*

IL-17A Ab

BNx

91±25 (4)*+

96±5 (4)*+

1.19±0.03 (4)*

2.12±0.19 (4)*

IgG Ab

RIR

412±28 (4)*

319±28 (4)*

1.15±0.05 (4)*

2.37±0.06 (4)*

TNF-α Ab

RIR

207±25 (4)*+

243±7 (4)*+

0.55±0.06 (4)+

0.90±0.05 (4)*+

IL-6 Ab

RIR

217±35 (4)*+

222±20 (4)*

0.68±0.04 (4)*+

0.99±0.13 (4)*+

IL-17A Ab

RIR

93±8 (4)*+

78±6 (4)*+

0.61±0.04 (4)*+

1.15±0.06 (4)*+

IgG Ab

HIR

10296±793 (6)*

10887±503 (6)*

0.65±0.05 (6)*

0.97±0.05 (6)*

TNF-α Ab

HIR

9133±1668 (4)*

4703±1040 (4)*+

0.71±0.04 (4)*

0.69±0.04 (4)*+

IL-6 Ab

HIR

10155±2173 (4)*

3680±236 (4)*+

0.73±0.06 (4)*

0.77±0.03 (4)*+

IL-17A Ab

HIR

ND

2726±305 (4)*+

ND

0.72±0.04 (4)*+

IgG Ab

BNx+HIR

14649±837 (6)*$

15021±2074 (6)*$

2.02±0.07 (6)*$

2.65±0.20 (6)*$

TNF-α Ab

BNx+HIR

6202±1579 (4)*+

6066±623 (4)*+

1.80±0.17 (4)*

2.70±0.08 (4)*

IL-6 Ab

BNx+HIR

6407±1754 (4)*+

5384±582 (4)*+

1.81±0.36 (4)*

2.57±0.20 (4)*

IL-17A Ab

BNx+HIR

ND

4007±1142 (4)*+

ND

2.99±0.11 (4)*

IgG Ab

RIR+HIR

21346±2742 (4)*$

17311±1689 (4)*$

1.24±0.08 (4)*$

2.15±0.06 (4)*$

TNF-α Ab

RIR+HIR

12361±1196 (7)*+

9542±906 (7)*+

0.66±0.02 (7)*+

1.20±0.15 (7)*+

IL-6 Ab

RIR+HIR

12237±1318 (6)*+

10212±1309 (6)*+

0.84±0.05 (6)*+

1.32±0.16 (6)*+

IL-17A Ab

RIR+HIR

ND

2331±996 (4)*+

ND

1.28±0.16 (4)*+

  1. ND, not determined; Ab, antibody; KO, knockout mice; HIR, hepatic ischemia and reperfusion; TNF-α, tumor necrosis factor alpha; IL-6, interleukin 6; IL-17A, interleukin 17A; IL17R, interleukin 17A receptor; IgG Ab, IgG isotype control antibody.
  2. The data are presented as mean±s.e.m. *P<0.05 vs sham-operated mice; #P<0.05 vs C57 (WT) mice; +P<0.05 vs IgG isotype control antibody-treated mice; $P<0.05 vs HIR alone.