Figure 3
From: High-mobility group box-1 protein promotes granulomatous nephritis in adenine-induced nephropathy
Increased expression of HMGB1 and MCP-1 in the urine and serum of granulomatous nephritis rats. (a) Western blotting shows increased HMGB1 levels in urine (5 or 10 ml samples concentrated using heparin beads) over time (left panel) and at different doses (right panel) in rats fed adenine. Urine HMGB1 levels peaked at 12 weeks. The protein signals are expressed as the ratio of the densitometric intensity of the urine HMGB1 concentration, which was normalized to urine creatinine. (b) ELISA shows increased urine MCP-1 levels in rats that were fed different doses of adenine for 5 weeks. n=3–15; **P<0.01. (c–e) ELISA shows increased serum HMGB1 in rats fed 0.25% adenine for 20 weeks (c), and a dose-dependent increase in serum MCP-1 (d) with a peak at 20 weeks in the time-dependent model (e). n=3–15; *P<0.05, **P<0.01.
