Figure 7
From: High-mobility group box-1 protein promotes granulomatous nephritis in adenine-induced nephropathy
Inflammatory changes and reduction in the number of macrophages in Hmgb1+/− mice with DHA crystal-induced nephritis. (a and b) In DHA crystal-induced nephritis mice, MCP-1 expression of Hmgb1+/− mice was significantly decreased in both whole kidney extracts and urine compared with that of WT mice. (c) Immunohistochemical analysis of macrophages in kidney tissues in adenine-fed mice and control mice. Macrophage aggregates were observed around the crystals (asterisks) in the kidney tissue of adenine-fed mice, whereas no crystals and few macrophages were seen in control mice. Black arrows indicate macrophages. Nuclei were counterstained with Mayer's hematoxylin. Original magnifications: × 400. (d) The number of macrophages (stained by rat anti-mouse mAb) was also decreased in adenine-fed Hmgb1+/− mice compared with that in adenine-fed WT mice. n=3–7; *P<0.05, **P<0.01.
