Figure 3
WZ4002-resistant (WR) cells depend on Pin1 for survival. (a; Upper panel) Effects of Pin1 knockdown on cell viability of H1975 parental cells and WR7 cells. Cells were transfected with negative control (NC) siRNA (10 nM) or Pin1-specific siRNA (Ambion #10546, 10 nM) and cultured for 24 h, and cell viability was assessed for another 72 h. Results are expressed as the mean (n=3)±s.d. (lower panel). Crystal violet staining for viable cells. Cells (5.0 × 105) were seeded in 57-mm culture dish and transfected with NC siRNA (10 nM) or Pin1 siRNA (10 nM). The cells were cultured for 96 h, and then were fixed and stained with Crystal violet. (b) Western blots examining the effects of Pin1 depletion in parental and WR7 cells. Cells transfected with NC siRNA (10 nM) or Pin1 siRNA (Ambion #10546, 10 nM) were cultured for 72 h. (c) Caspase 3/7 activity in parental and WR7 cells. Cells transfected with NC siRNA (10 nM) or Pin1 siRNA (Ambion #10546, 10 nM) were cultured for 72 h, and caspase activity was evaluated. Columns, mean (n=3); bars, s.d. **P<0.01. EL, extra long; L, long; S, short.
