Figure 2
H56/CAF01 sustains population of IL-2 and IL-17-producing vaccine-specific CD4 T cells in lung parenchyma during Mtb infection. CB6F1 mice were assayed before and after aerosol Mtb infection using i.v. staining to identify parenchymal (IV–) T cells. Lung cells were stimulated ex vivo with ESAT-61-15 (a) or TB10.471-88 (b) and stained by ICS to determine the total number of antigen-specific CD4 cells (IFNγ, TNFα, IL-2, or IL-17-producing). (c) The total number of lung parenchyma (IV-) ESAT-6-specific CD4 T cells expressing the indicated cytokine from adjuvant control (Ctrl,○) and H56/CAF01-vaccinated (●) mice is shown . Symbol, mean±s.e.m. of 3–6 mice. *P< 0.05, **P<0.01 ***P<0.001 vs. Ctrl. (d) Frequency of IV–ESAT-6-specific Lung CD4 T cells expressing each cytokine combination, based on combinatorial Boolean gating analysis, in adjuvant control (Ctrl, □) and H56/CAF01-vaccinated (▪) mice 42 days post Mtb infection. Bar, mean±s.d. of five(□) or six(▪) mice.
