Figure 3

H56/CAF01 promotes a vaccine-specific population of KLRG1-CXCR3+ CD4 T cells in the lung parenchyma and vasculature. CB6F1 mice chronically infected with Mtb were injected i.v. with anti-CD45-FITC prior to tissue harvest to characterize parenchymal (IV–) and intravascular (IV+) lung CD4 T cells in adjuvant control (Ctrl) and H56/CAF01-vaccinated (H56) mice. (a) Representive plots of KLRG1 expression and IV labelling of I-A^d:TB10.4_74-88 tetramer+ (left) and I-A^b:ESAT-6_4-17 tetramer+ (right) CD4 lung cells from a control (top) and H56/CAF01-vaccinated (bottom) mouse 6 wks post infection. (b) Compiled %KLRG1+ of I-A^d:TB10.4_74-88 and I-A^b:ESAT-6_4-17 tetramer+ CD4 T cells from the lung parenchyma (IV–, left) and vasculature (IV+, right) from (a). Bar, mean±s.d. of 4–6 mice with >15 tetramer+ events. *p<0.05, ***p<0.001 by paired t-test. (c–d) Surface staining of I-A^b:ESAT-6_4-17 tetramer+lung CD4 T cells from 14 wk Mtb-infected adjuvant control (top) and H56/CAF01-vaccinated (bottom) mice, located in the tissue parenchyma (IV–, left) and vasculature (IV+, right). Plots are electronically concatenated from four mice, and % expression of individual mice plotted below. Bar, mean±s.d. **P<P< 0.01, ****P<0.0001 by t-test. All data representative of ⩾2.

PowerPoint slide