Figure 5
Vaccine-specific lung vasculature CD4 T cells from H56/CAF01-vaccinated mice efficiently traffic into the Mtb-infected lung parenchyma. (a) Schematic of adoptive transfer system to study the parenchymal homing ability of lung-circulating H56/CAF01-induced CD4 T cells. Total IV+ lung CD4 T cells were FACS-sorted from 7week Mtb-infected adjuvant control or H56/CAF01-immunized BL/6 mice (CD45.2) and transferred into infection-matched congenic recipients (CD45.1). After 14 h, the migration of donor cells into the lung parenchyma was determined by a second i.v. stain of recipients prior to lung cell isolation and surface staining. (b) KLRG1 expression of ESAT-64-17 tet+ cells from sorted IV+ donor CD4 T cells from individual adjuvant control (left) or H56/CAF01-vaccinated (right) mice prior to transfer. Bar, mean±SD of 4 mice. (c) KLRG1 expression and IV-labeling of CD45.2+ I-Ab:ESAT-64-17 tetramer+ donor CD4 T cells isolated from the lungs of recipient mice to assess trafficking and phenotype of transferred cells. Bar, mean±s.d. of 3–4 mice. Data representative of two experiments with similar results. *P<0.05, **P<0.01 by unpaired t-test.
