Table 1 Panel of antibodies used in this study
Antigen | Clone | Dilution | Antigen retrieval | Source |
|---|---|---|---|---|
AMACR | Polyclonal | 1:100 | Pressure cooker | Zeta, Arcadia, CA, USA |
Carbonic anhydrase IX | Polyclonal | 1:1000 | Pressure cooker | Abcam, Cambridge, MA, USA |
Cathepsin K | 3F9 | 1:500 | Pressure cooker | Abcam |
CD10 | 56c6 | 1:20 | Pressure cooker | Vector Laboratories, Burlingame, CA, USA |
Chromogranin | LK2H10 | 1:4000 | Pressure cooker | Thermo Scientific, Waltham, MA, USA |
CK | AE1/AE3 | 1:200 | 10 min proteinase | Dako, Carpinteria, CA, USA |
CK7 | OV-TL 12/30 | 1:2000 | 10 min proteinase | Dako |
CK 7/8 | CAM5.2 | 1:50 | 10 min proteinase | BD Biosciences, San Jose, CA, USA |
EMA | E29 | 1:200 | None | Dako |
HNF1B | Polyclonal | 1:400 | Pressure cooker | Sigma, St Louis, MO, USA |
Kidney-specific cadherin | MRQ-33 | Prediluted | Heat, CC1 buffer | Cell Marque, Rocklin, CA, USA |
KIT | Polyclonal | 1:250 | None | Dako |
PMEL | HMB45 | 1:400 | None | Dako |
RCC | SPM314 | 1:400 | Pressure cooker | Dako |
S100A1 | Polyclonal | 1:3000 | Pressure cooker | Abcam |
SDHA | 2E3 | 1:5000 | Pressure cooker | Abcam |
SDHB | 21A11 | 1:200 | Pressure cooker | Abcam |
Synaptophysin | Polyclonal | 1:50 | None | Invitrogen, Grand Island, NY, USA |
TFE3 | MRQ37 | 1:500 | Pressure cooker | Cell Marque |
Vimentin | Vim 3B4 | 1:400 | 20 min proteinase | Dako |
