Extended Data Figure 9: In vitro lymphopoiesis in the presence of ApoM+HDL generates B cells at different stages of development.
From: HDL-bound sphingosine-1-phosphate restrains lymphopoiesis and neuroinflammation
a, Phenotyping of B220+ cell populations generated from wild-type (WT) Lin− bone marrow after 8 days of culture in methylcellulose medium. Initially, cells were gated as B220−, B220+, or B220hi. From populations expressing B220, pro-B cell (Hardy fractions B/C) and pre-B cell (Hardy fraction D) equivalents were identified. Pro-B cells were defined as IL-7Rhi, CD19+, cKit+, CD43+ and IgM−. Pre-B cells were defined as IL-7R+, CD19+, cKit−, CD43− and IgM−/lo. b, Western blot analysis of two batches of mouse HDL (batches n1 and n2) isolated from wild-type or Apom−/− mice showing the absence of contaminating albumin. c, LC-MS/MS analysis of HDL isolated from pooled plasmas of wild-type or Apom−/− mice. *P < 0.05, **P < 0.005. n = 3. dhSph, dihydrosphingosine; dhS1P, dihydro-sphingosine-1-phosphate; Sph, sphingosine.
