Supplementary Figure 15: SPADE trees of CyTOF data collected during a single experiment.

Cells isolated from spleens of mice treated with drug (clioquinol) and vehicle control (PEG400). Trees show the lymphocyte lineage of CD45+ cells. Key for trees shown to orient toward CD4+ and CD8+ cells. Arrows highlight CD45+CD3+CD4–CD8+Thy1.2+TCRb+CD62LhiCD44lo population increase after clioquinol treatment. Horizontal color scales represent median intensity (blue = low, red = high) for marker indicated.