Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Correspondence
  • Published:

Validation of AAV-mediated gene targeting

Nature Biotechnology volume 20, page 658 (2002)Cite this article

To the editor:

A series of letters to Nature Biotechnology have highlighted the controversy surrounding chimeric RNA/DNA oligonucleotide (RDO) technology1,2,3, including a failed attempt to modify the human, X-linked hypoxanthine phosphoribosyltransferase (HPRT) locus that established specific criteria for validating gene-targeting experiments3. We believe an alternative gene-targeting approach based on single-stranded parvoviral vectors, such as adeno-associated virus (AAV), satisfies these criteria.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

  • Purchase on SpringerLink
  • Instant access to the full article PDF.

USD 39.95

Prices may be subject to local taxes which are calculated during checkout

References

  1. van der Steege, G. et al. Nat. Biotechnol. 19, 305–306 (2001).

    Article  CAS  Google Scholar 

  2. Graham, I.R., et al. Nat. Biotechnol. 19, 507–508 (2001).

    Article  CAS  Google Scholar 

  3. Albuquerque-Silva, J., Vassart, G., Lavinha, J. & Abramowicz, M.J. Nat. Biotechnol. 19, 1011 (2001).

    Article  CAS  Google Scholar 

  4. Russell, D.W. & Hirata, R.K. Nat. Genet. 18, 325–330 (1998).

    Article  CAS  Google Scholar 

  5. Inoue, N., Hirata, R.K. & Russell, D.W. J. Virol. 73, 7376–7380 (1999).

    CAS  PubMed  PubMed Central  Google Scholar 

  6. Hirata, R.K. & Russell, D.W. J. Virol. 74, 4612–4620 (2000).

    Article  CAS  Google Scholar 

  7. Inoue, N., Dong, R., Hirata, R.K. & Russell, D.W. Mol. Ther. 3, 526–530 (2001).

    Article  CAS  Google Scholar 

  8. Brown, J.P., Wei, W. & Sedivy, J.M. Science 277, 831–834 (1997).

    Article  CAS  Google Scholar 

  9. Yanez, R.J. & Porter, A.C. Gene Ther. 5, 149–159 (1998).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medicine, University of Washington, Seattle, 98195, WA

    David W. Russell & Roli K. Hirata

  2. Centers for Disease and Control and Prevention, Atlanta, 30333, GA

    Naoki Inoue

Authors
  1. David W. Russell
    View author publications

    Search author on:PubMed Google Scholar

  2. Roli K. Hirata
    View author publications

    Search author on:PubMed Google Scholar

  3. Naoki Inoue
    View author publications

    Search author on:PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Russell, D., Hirata, R. & Inoue, N. Validation of AAV-mediated gene targeting. Nat Biotechnol 20, 658 (2002). https://doi.org/10.1038/nbt0702-658

Download citation

  • Issue date:

  • DOI: https://doi.org/10.1038/nbt0702-658

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing