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Chimeraplasty validation

Nature Biotechnology volume 19, page 1011 (2001)Cite this article

To the editor

Initial studies of gene correction using chimeric RNA/DNA oligonucleotides (RDOs), also termed chimeraplasts, reported exciting rates of correction of point mutations (up to 40% in one in vivo model1). However, the technique has not yet fulfilled its promise in the fields of transgenics and gene therapy, and some groups have reported persistent failures2,3. In this context, high hopes for the outstanding potential of the technique were raised again when a group of independent researchers recently reported success4,5. We believe, however, it is wise not only to share negative results, as Van der Steege et al.3 highlighted in the April issue, but also to propose criteria for further experiments intending to validate RDO technology.

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Authors and Affiliations

  1. Laboratoire de Génétique Médicale–IRIBHN and Hôpital Erasme, Université Libre de Bruxelles (ULB), 808 Lennik St., Brussels, B-1070, Belgium

    João Albuquerque-Silva, Gilbert Vassart & Marc J. Abramowicz

  2. Instituto Nacional de Saúde Dr. Ricardo Jorge, Av. Padre Cruz, Lisbon, 1649-016, Portugal

    João Albuquerque-Silva & João Lavinha

Authors
  1. João Albuquerque-Silva
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  2. Gilbert Vassart
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  3. João Lavinha
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  4. Marc J. Abramowicz
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Albuquerque-Silva, J., Vassart, G., Lavinha, J. et al. Chimeraplasty validation. Nat Biotechnol 19, 1011 (2001). https://doi.org/10.1038/nbt1101-1011a

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