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GFP movement between chloroplasts

Nature Biotechnology volume 17, page 1146 (1999)Cite this article

To the editor:

In your September issue (Nat. Biotechnol. 17, 906), we described the presence of green fluorescent protein (GFP) in multiple chloroplasts in a single cell following injection of a gfp gene construct into a single chloroplast1, and suggested that GFP was able to move through stromules (interconnecting stroma tubules)2 from the injected chloroplast to adjacent chloroplasts. In an accompanying Research News and Views article, Henry Daniell3 drew attention to the lack of evidence for stromules containing GFP in transplastomic tobacco and rice plants expressing a gfp-aadA gene-fusion following microprojectile bombardment4 and observed that stromules had not yet been experimentally demonstrated in transplastomic plants expressing gfp stably integrated within the chloroplast genome. He also raised the question whether movement of proteins through stromules occurred naturally in plants.

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References

  1. Knoblauch, M. et al. Nat. Biotechnol. 17, 906– 909 (1999).

    Article  CAS  Google Scholar 

  2. Köhler, R.H. et al. Science 276, 2039–2042 (1997).

    Article  Google Scholar 

  3. Daniell, H. Nat. Biotechnol. 17, 855–856 (1999).

    Article  CAS  Google Scholar 

  4. Khan, M.S. & Maliga, P. Nat. Biotechnol. 17, 910–915 (1999).

    Article  CAS  Google Scholar 

  5. Hibberd, J.M. et al. Plant J. 16, 627–632 (1998).

    Article  CAS  Google Scholar 

  6. ONeill et al. Plant J. 3, 729–738 ( 1993).

    Article  CAS  Google Scholar 

  7. Tirlapur, U.K. et al. Eur. J. Cell Biol. 78, 233– 240 (1999).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. University of Cambridge, Cambridge, UK

    John C. Gray, Julian M. Hibberd & Philip J. Linley

  2. Nunhems Zaden BV, Haelen, 6080 AA, The Netherlands

    Bert Uijtewaal

Authors
  1. John C. Gray
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  2. Julian M. Hibberd
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  3. Philip J. Linley
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  4. Bert Uijtewaal
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Gray, J., Hibberd, J., Linley, P. et al. GFP movement between chloroplasts. Nat Biotechnol 17, 1146 (1999). https://doi.org/10.1038/70653

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