Figure 1: FAM126 is a component of the PI4KIIIα complex.

(a) Volcano plots of proteins associating with TTC7B–GFP versus GFP alone (top) and FAM126A–GFP versus GFP alone (bottom), from a label-free proteomics analysis of anti-GFP immunoprecipitates of HEK 293T cells stably expressing TTC7B–GFP, FAM126A–GFP or GFP alone. The logarithmic ratios of protein intensities are plotted against negative logarithmic P values of two-tailed Student’s t-test, equal variance, performed from n = 3 independent experiments. The red dashed line (significance, 0.05) separates specifically interacting proteins (top right portion of plot) from background. Selected top hits are indicated with black dots (bait is indicated in green), and all specific interactors are reported in Supplementary Table 1. (b) Immunoblot analysis of PI4KIIIα complex components in anti-GFP immunoprecipitates of HEK 293T cells stably expressing TTC7B–GFP (top), FAM126A–GFP (bottom) or GFP alone. (c) Domain structure, evolutionary conservation and predicted extent of disordered secondary structural elements of FAM126. (d) Immunoblot analysis of anti-GFP immunoprecipitates from HeLa cells transfected with 3xFLAG–PI4KIIIα, TTC7B–MYC, EFR3B–HA, and the indicated GFP-tagged construct. Arrows denote specific bands and arrowheads denote nonspecific antibody bands. Unprocessed original scans of blots are shown in Supplementary Fig. 6.