Supplementary Figure 2: FAM126A is a soluble, cytosolic protein that is recruited to the plasma membrane by EFR3B and TTC7B. | Nature Cell Biology

Supplementary Figure 2: FAM126A is a soluble, cytosolic protein that is recruited to the plasma membrane by EFR3B and TTC7B.

From: The leukodystrophy protein FAM126A (hyccin) regulates PtdIns(4)P synthesis at the plasma membrane

Supplementary Figure 2

COS-7 cells were transfected with the indicated combinations of the following plasmids: GFP-FAM126A-N, FAM126A-GFP, soluble mCherry (mCherry), a plasma membrane-targeted mCherry containing the first 11 residues of Lyn kinase (PM-mCherry) either alone (a) or in combination with EFR3B-HA and TTC7B-mTagBFP (b). The cells were imaged live by confocal microscopy; shown are single z-plane images. Scale bars, 20 μm.

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