Supplementary Figure 3: Effects of the ablation of FRCs on T cells and B cells.
From: B cell homeostasis and follicle confines are governed by fibroblastic reticular cells
(a,b) Lymph nodes from Ccl19-Cre x iDTR mice were collected and processed into single cell suspensions. CD4+ and CD8+ T cells were enumerated using flow cytometric analysis (CD45+B220–CD3+CD4+/–CD8+/–). Data are representative of at least three independent experiments (mean+/-sd, n=4 mice/group per experiment). (c-e) Schematic representation of the experimental protocols utilized to assess T cell immunity. (f) Ccl19 promoter activity was evaluated in B cells from Ccl19-Cre x Rosa26-EYFP mice. (g) B cells from DTR+Cre+ mice were cultured in vitro in the presence of DTxn and viability was determined over the course of 72 h (n=3 wells/group). NS non significant, * p<0.01, **p<0.001
